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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Cancer Res. 2014 Aug 29;74(18):5032–5044. doi: 10.1158/0008-5472.CAN-13-3390

Figure 1. HD breast tissues have increased basal γH2AX and shortened telomeres compared to LD breast tissues.

Figure 1

γH2AX protein levels and telomere content assessed by immunohistochemistry and FISH, respectively, in serial paraffin breast tissue sections from 13 LD and 14 HD disease-free women. (A) Left: Representative bright field images (20X original magnification) of paraffin sections from 12 LD and 14 HD tissues stained for γH2AX (brown). Right: Average and SEM of γH2AX intensity per cell. (B) Left: Representative fluorescent images (63X original magnification) of paraffin sections from 12 LD and 11 HD tissues stained for telomere (red), centromere (green) and DNA (DAPI, grey). S: stromal cells, E: epithelial cells. Right: Average and SEM of telomere signal/centromere signal per epithelial cell nucleus.