FIG 2.
cFP reduces proinflammatory cytokine levels in mouse macrophages stimulated with LPS. (A) J774A.1 cells were stimulated with LPS (100 ng/ml) for 48 h in the presence of the indicated concentrations of cFP. The levels of TNF-α and IL-6 in the culture supernatants were measured by ELISA. Data represent means ± SD (n = 4). Statistical differences between groups were analyzed by using Student's t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (B) J774A.1 cells were stimulated with LPS (100 ng/ml) for the indicated numbers of hours in the presence or absence of 4 mM cFP. Relative mRNA levels of Tnf and Il6 were measured by qRT-PCR and normalized to the Hprt mRNA level. Data represent means ± SD (n = 3). Statistical analyses were performed by using Student's t test (***, P < 0.001).