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. Author manuscript; available in PMC: 2015 Feb 17.
Published in final edited form as: Free Radic Biol Med. 2009 Jun 12;47(6):750–759. doi: 10.1016/j.freeradbiomed.2009.06.010

Figure 8. shRNA-mediated down-regulation of hOGGl mRNA reduces mitochondrial aconitase and augments oxidant-induced apoptosis.

Figure 8

Cells were transfected with mock, control shRNA or hOGGl shRNA for 24 h as delineated in the Experimental Procedures. As compared to mock shRNA controls, shRNA against hOGGl reduces OGG1 mRNA levels by ~90% (n=6) (A) and decreases mitochondrial aconitase levels by ~30% (n=3) (B). As compared to Mock shRNA-treated controls (Con), DNA fragmentation caused by asbestos (5–25 µg/cm2) or H2O2 (100 µM) for 24h was augmented by shRNA against hOGGl (n=6); data expressed as fold control (mock shRNA-treated) (C) * p < .05 v. Control; † p < .05 v. Mock shRNA.