Figure 6. Mitochondrial wild-type and long alpha/beta 317–323 hOggl mutant co-precipitate with mitochondrial aconitase.
Mitochondrial proteins obtained from control (Con) and mt-hOggl (wild-type [WT] and long α/β 317–323 [Laβ]) overexpressing A549 cells were subjected to Western blot analysis before and after immunoprecipitation (IP) using antimyc performed as described in the Experimental Procedures. As expected, mitochondrial proteins probed before IP demonstrated hOgg 1 and c-myc only in the hOgg 1 overexpressing cells (either wild-type or Lαβ mutant hOggl) despite similar levels of mitochondrial aconitase and COX IV as control cells. After IP with c-myc, mitochondrial aconitase and hOggl, but not COX IV, co-precipitated with the myc constructs containing either wild-type or Lαβ mutant hOggl.