Figure 2.
An overview of the methodology for the generation of iPSCs. (1) Establishment of culture: the source cells are cultured for further use as host cells for the delivery of reprogramming proteins. (2) The cultured source cells are then transfected with the four factors from Yamanaka's cocktail and incubated on feeder layers that provide the nourishment to host cells and are responsible for the formation of extra cellular matrix, under suitable conditions of media. Two types of methods for the delivery of reprogramming factors into the somatic cells can be used- Integrating viral vector systems and Non-Integrating methods. (3) After the formation of iPSCs, they are characterized by different morphological and physicochemical analyses, which is followed by the expansion of iPSCs.