Figure 3.
TLR3 is critical for CVB4-induced T cell-mediated insulitis in female NOD mice. Evaluation of T cell infiltration of pancreatic islets in 10-week-old uninfected and CVB4-infected female wild-type (WT) and TLR3 knockout (KO) NOD mice. Serial sections of pancreas stained for insulin and the T cell markers CD4 and CD8 revealed that T cells were abundant in CVB4-infected wild-type NOD islets but scarce in CVB4-infected TLR3 KO NOD islets. No differences in T cell infiltration of islets of uninfected wild-type and TLR3 KO NOD mice were observed. At least 4 mice per group were analyzed. Pictures were taken at ×20 (A–D) or ×100 (E and F) magnification. Scale bars in A–D indicate 500 μm, whereas scale bars in E and F indicate 50 μm. Images in this figure show isolated representative data on sections rather than summary data of all of the mice.