Fig 4.
Hypoxia and a microdosage stromal cell-derived factor-1α (SDF-1α) augmented the surface expression of cognate receptor CXC chemokine receptor 4 (CXCR4) on mesenchymal stem cells (MSCs). A. After treatment with hypoxia for 0, 6, 12, 24, 48 and 72 hours, expression of CXCR4 was detected by western blotting. *; P<0.05, #; P<0.01 vs. control. B. MSCs were incubated with SDF-1α (10 ng/mL) or AMD3100 (5 μg/mL); expression of CXCR4 was detected by western blotting. C. The results of CXCR4 expression detected by flow cytometry. D. Quantification of flow cytometry results.