Table 1. Factors secreted by PSCs and normal MRC5 fibroblasts.
Supernatants were collected from hPSC and MRC5 monocultures and the levels of cytokines were measured using Multiplex suspension array. The relative expression of each factor was calculated by relating the average concentration in hPSC supernatants to that of MRC5 supernatant after culturing for 24 hr (n = 4). Factors highlighted in bold were significantly increased in supernatants from hPSC compared to those from MRC5. p, p-value; NA, ratio was not applicable (see Suppl. Table 1). t-test followed by Bonferroni's post-test: *, p < 0.05; **, p < 0.001; ns, not significant.
| Analyte | hPSC vs. MRC5 relative expression | p | Analyte | hPSC vs. MRC5 relative expression | p | Analyte | hPSC vs. MRC5 relative expression | p |
|---|---|---|---|---|---|---|---|---|
| Interleukins | Chemokines/monokines | Growth factors | ||||||
| IL-8 | 60.58 | ** | GROα | 103.43 | ** | M-CSF | 14.65 | ns |
| IL-6 | > 31.31 | * | Eotaxin | 46.22 | ** | G-CSF | 7.19 | ns |
| LIF | 8.28 | ** | SDF-1α | 37.92 | ** | PDGF-BB | 6.73 | ns |
| IL-1Rα | 6.25 | ** | IP-10 | 8.70 | * | GM-CSF | 5.37 | ns |
| IL-15 | 4.87 | ** | MCP-3 | 5.99 | ns | HGF | 2.19 | |
| IL-2 | 4.68 | ns | RANTES | 3.40 | FGF basic | 2.16 | ||
| IL-17 | 4.17 | ns | MIP-1α | 2.31 | b-NGF | 1.83 | ||
| IL-4 | 3.88 | MIF | 1.74 | SCF | 1.51 | |||
| IL-2Rα | 2.91 | MIG | 1.60 | VEGF | 1.39 | |||
| IL-16 | 2.22 | CTACK | 1.53 | SCGF-b | 1.03 | |||
| IL-9 | 2.20 | MCP-1 | NA | Apoptosis Mediator | ||||
| IL-12(p40) | 2.18 | MIP-1β | NA | TRAIL | 2.71 | |||
| IL-1a | 2.14 | Inflammatory cytokines | Adhesion molecules | |||||
| IL-3 | 1.96 | IFN-γ | 6.91 | ns | ICAM-1 | 3.42 | ||
| IL-7 | 1.83 | TNF-α | 4.63 | ns | VCAM-1 | 1.92 | ||
| IL-18 | 1.71 | TNF-β | 1.71 | |||||
| IL-12(p70) | 1.31 | IFN-α2 | 1.61 | |||||
| IL-10 | 1.29 | |||||||
| IL-13 | 1.11 | |||||||
| IL-1β | NA | |||||||
| IL-5 | NA | |||||||