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. 2015 Jan 14;5:7767. doi: 10.1038/srep07767

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The upper panel shows an example of the gating strategies for CD-1 lymphocytes in lower panel. Thymic CD4⁺CD8⁻CD25⁻Foxp3⁺ cells of CD-1 mice were further analysed for individual expression of Nrp1 and Helios in thymus and thymic glands of NOD and C57BL/6 mice were examined similarly (lower panel). Results are expressed as means ± SEM, from two experiments (n = 4–5 mice/experiment). *** denotes p < 0.001, unpaired t-tests were performed for comparisons.