Figure 6. Splicing-dependent NMD reporter in developing Xenopus embryos.
(a) Fluorescence microscopy of representative embryos expressing pNMD+ (upper panels) or pNMD− (bottom panels). Embryos were photographed in white light (upper rows) and the green (middle rows) and red (bottom rows) fluorescence channels. All embryos were imaged under the same settings, except those at stage 45, for which the exposures were decreased to avoid signal saturation. Scale bar: 1 mm. (b) The change of NMD activity during Xenopus development. Data for 7 embryos in 3 independent experiments are shown as a box plot (mean, median, standard deviation, and extrema).