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Published in final edited form as: Nat Med. 2011 Oct 30;17(11):1481–1489. doi: 10.1038/nm.2513

Effects of IL-6 on GLP-1 secretion in GLUTag cells. (a) Western blots of IL-6 receptor (top, with HeLa cell extract as the positive control (ctrl)), pSTAT3 (middle) and actin (bottom). (b) GLP-1 secretion after 15 min of stimulation with IL-6 and 0.1 mM glucose; we used 1 μM glucose-dependent insulinotropic peptide (GIP) as the positive control (n = 5). (c) Capacitance traces following stimulation with IL-6 (top left). Average capacitance following 15 min of stimulation with or without IL-6 (top right, n = 6–11). Ca²⁺ current following stimulation with or without IL-6 (bottom left, n = 6–11). Average capacitance following 15 min of stimulation with or without IL-6 and AG490 (bottom right, n = 5–9). (d) GLP-1 secretion in response to 2 h of stimulation with 11 mM glucose after 24 h with or without IL-6 (left, n = 3). GLP-1 content after 24 h with or without IL-6 (middle, n = 6). GLP-1 secretion in response to 2 h of stimulation with 11 mM glucose after 0–24 h with or without IL-6 (right, n = 3). (e) GLP-1 secretion in response to 2 h of stimulation with 11 mM glucose after 24 h with or without IL-6 (left, n = 3). GLP-1 content after 24 h with or without IL-6 (right, n = 3). (f) GLUTag mRNA in response to IL-6; data are expressed as a fold of the untreated control (broken line) (n = 3–6). (g) GLUTag mRNA after 24 h with or without IL-6 (n = 3). (h) 2-deoxy-³H-D-glucose (³H-2dG) uptake after 24 h with or without IL-6 (n = 4). (i) GLP-1 secretion in response to 2 h of stimulation with 11 mM glucose after 24 h with or without IL-6 (n = 3). (j) GLP-1 secretion in response to 2 h of stimulation with 0.1 or 11 mM glucose after 24 h with or without IL-6 (n = 6). Data represent means ± s.e.m. *P < 0.05, determined by ANOVA.

Effects of IL-6 on GLP-1 secretion in GLUTag cells. (a) Western blots of IL-6 receptor (top, with HeLa cell extract as the positive control (ctrl)), pSTAT3 (middle) and actin (bottom). (b) GLP-1 secretion after 15 min of stimulation with IL-6 and 0.1 mM glucose; we used 1 μM glucose-dependent insulinotropic peptide (GIP) as the positive control (n = 5). (c) Capacitance traces following stimulation with IL-6 (top left). Average capacitance following 15 min of stimulation with or without IL-6 (top right, n = 6–11). Ca²⁺ current following stimulation with or without IL-6 (bottom left, n = 6–11). Average capacitance following 15 min of stimulation with or without IL-6 and AG490 (bottom right, n = 5–9). (d) GLP-1 secretion in response to 2 h of stimulation with 11 mM glucose after 24 h with or without IL-6 (left, n = 3). GLP-1 content after 24 h with or without IL-6 (middle, n = 6). GLP-1 secretion in response to 2 h of stimulation with 11 mM glucose after 0–24 h with or without IL-6 (right, n = 3). (e) GLP-1 secretion in response to 2 h of stimulation with 11 mM glucose after 24 h with or without IL-6 (left, n = 3). GLP-1 content after 24 h with or without IL-6 (right, n = 3). (f) GLUTag mRNA in response to IL-6; data are expressed as a fold of the untreated control (broken line) (n = 3–6). (g) GLUTag mRNA after 24 h with or without IL-6 (n = 3). (h) 2-deoxy-³H-D-glucose (³H-2dG) uptake after 24 h with or without IL-6 (n = 4). (i) GLP-1 secretion in response to 2 h of stimulation with 11 mM glucose after 24 h with or without IL-6 (n = 3). (j) GLP-1 secretion in response to 2 h of stimulation with 0.1 or 11 mM glucose after 24 h with or without IL-6 (n = 6). Data represent means ± s.e.m. *P < 0.05, determined by ANOVA.