Figure 1.
Structural conservation of the C-terminal 4Fe-4S cluster-forming cysteines of Cas4-like proteins from Campylobacter phages. (A) The predicted structure of the bacteriophage CP8 Cas4-like protein monomer from Phyre2 indicating the amino acid positions of the four conserved cysteine residues that form a 4Fe-4S cluster; (B) Arrangement of the monomer structures within the decameric toroidal Cas4 protein (SsO0001) of S. solfataricus showing positions of the 4Fe-4S clusters (13); (C) Phylogenetic analysis using a neighboring-joining tree without distance correction (ClustalW2) revealing lated family of proteins where Class III phage Cas4-like proteins cluster with C. fetus, C. concisus and SsO0001.
