Figure 3. Intramembranous ossification is impaired in Runx2 deficient mice.
(A) Dorsal view of Alizarin red/Alcian blue stained skulls from newborn littermates is shown. Dotted lines mark the borders of calcification in occipital bones and open fontanels at the sagittal plane. (B) Coronal sections from rostral part of newborn calvaria were stained with H&E. Frontal bones are shown at 10× magnification. Double arrows indicate thickness of the frontal bone in the boxed region. (C) Calvarial thickness was determined at 6 randomly selected areas along the frontal bone. Pooled data from 3 litters is shown in graph. Statistical significance was calculated by Student’s t test. Asterisks represent p < 0.05. Scale bars are 100 µm.