Table 2.
Primers
| Primer | Primer sequence (5′→3′) | Template target |
|---|---|---|
| RHL101 | TGAACGGGCTTTTAATCTGG | Ppa-obi-1 gDNA |
| RHL102 | GTGTCGAGATAGTCGGGCAT | Ppa-obi-1 gDNA |
| RHL104 | ATGACTGCTCCAAAGAAG | Ppa-obi-1 cDNA |
| RHL107 | ATTTGCCCTTCGTTCCAC | Ppa-obi-1 for RNAi |
| RHL108 | CTGTCTCAGTATGCCGAATG | Ppa-obi-1 for RNAi |
| RHL109 | ATTCAGCCGTGTTACAAATC | Ppa-obi-1 cDNA |
| RHL110 | CTGCATGTGTTCCGGTCTCG | Ppa-obi-1 cDNA |
| RHL113 | AAACCTCTGACACATGCAGC | Ppa-obi-1 cDNA |
| RHL121 | AGTTTCAAAGTTGCATGG | Ppa-obi-1 promoter |
| RHL124 | CTTCTTTGGAGCAGTCATGTAGCGATAATCAGGAGT | gfp; Ppa-obi-1 promoter |
| RHL132 | ATGACTGCTCCAAAGAAG | gfp |
| RHL136 | CAACCGTGTGCAGTAGAACC | Ppa-obi-1 5′ RACE |
| RHL149 | ACTATCCTATCATCGGAAGC | Ppa-obi-1 promoter |
| RHL151 | AGGGCATTGACAAATCATCG | Ppa-obi-1 cDNA |
| RHL154 | CACGACGTTGTAAAACGACG | Ppa-obi-1 cDNA |
| RHL169 | CGCGCTAAACCAATTCCGCC | C06G1.1 promoter |
| RHL181 | GTTGAGAGAAGAGGTGGAGTCC | C06G1.1 promoter |
| RHL187 | CTTCTTTGGAGCAGTCATCAGATCTGAAAATTTGACACATTG | gfp; C06G1.1 promoter |
| RHL267 | GGAACTAACATTAATGTCAC | C06G1.1 RNAi |
| RHL268 | GAGAAGTGCATAGTTGATG | C06G1.1 RNAi |
| RHL477 | GCGCTACAGGGGATTTTGTC | T02B11.3 promoter |
| RHL480 | TGAAAGATGCAAGTAAAGGAGAAGAACTTTTC | p95.75 GFP |
| RHL481 | AAGGGCCCGTACGGCCGACTA | p95.75 GFP |
| RHL482 | GTAGGAAACAGTTATGTTTGG | p95.75 GFP |
| RHL484 | CTTCAGAAAATGATCAGGAGTCTGGTTC | obi-1 cDNA |
| RHL487 | AGTGAAAAGTTCTTCTCCTTTACTTGCATCTTTCACGGAGTC | obi-1 cDNA; gfp |
| RHL490 | AACGAGAACCAGACTCCTGATCATTTTCTGAAGAAAGTTGAAAAAC | T02B11.3 promoter; obi-1 cDNA |
| RHL498 | GCGATAAGATCGGTCAATCTGAG | F16F9.3 promoter |
| RHL499 | GCCAGTAAGGGCTAGTAAGTG | F16F9.3 promoter |
| RHL500 | AACGAGAACCAGACTCCTGATCATATTTTGTTTCTTACTGTCTTG | F16F9.3 promoter |
| RHL501 | ATGGGTACGTTTCTGGGTATAG | T02B11.3 promoter; obi-1 cDNA |
| VR86 | GACTCCTGATTATCGCTACG | Ppa-obi-1 cDNA |
| VR87 | AGATAGTCAAACTATGCATC | Ppa-obi-1 cDNA |
| VR88 | CTAATAATCTACACTAAATG | Ppa-obi-1 cDNA |
| AG11112 | CTCGGAGGAGGAACTGGATC | Ppa-beta-tubulin RT-qPCR |
| AG11113 | GACCGTGTCAGAGACCTTAG | Ppa-beta-tubulin RT-qPCR |
| RH12548 | GCAGGAACATATATTCGCAG | Ppa-egl-4 RT-qPCR |
| RH12549 | TGTCACGGAACGTTTTGTAC | Ppa-egl-4 RT-qPCR |
| SL1 | GGTTTAATTACCCAAGTTTGAG | SL1 for 5′ RACE |
| SSLP21F | ACTGGTGCTCATCGCAAGA | Mapping marker |
| SSLP21R | CCTTCTGTTTTCCTACCCCC | Mapping marker |
| SSLP7F | TCTTGCATAACACCGAACAAA | Mapping marker |
| SSLP7R | AGGGCGTTACGTGAATAAGC | Mapping marker |
| SSLP17F | CAATGCAGTAGCCGAATCAT | Mapping marker |
| SSLP17R | CAATATTGGCCTTCACCCTG | Mapping marker |
| S447F | TGGGGATAGCGAAAATCATC | Mapping marker |
| S447R | CGCATCGTTATTCACGAAATTC | Mapping marker |
Letters in bold are based on different templates for fusion PCR.