Skip to main content
. Author manuscript; available in PMC: 2014 Dec 17.
Published in final edited form as: Cell Rep. 2014 Nov 20;9(5):1946–1958. doi: 10.1016/j.celrep.2014.10.058

Figure 4. Analysis of the ability of SAH peptides to disrupt p53-Mdm2 and p53-Mdm4 complexes in living cells, and antagonism by serum.

Figure 4

The Saos-2 p53-Mdm2 and p53-Mdm4 ReBiL cells in 96 well plates (20,000 cells per well) were pre-induced by doxycycline (500 ng/ml for 24 hr). At Time = 0, cells were washed with DMEM and treated with new media containing different PPI antagonists with or without 10% FBS. Luminescent signals were read every 5 minutes for 6 hr by Tecan-M200 at 37 °C. The ReBiL cells were treated with (A) Nutlin-3a and 10% FBS, (B) ATSP-7041 and 10% FBS, (C) Nutlin-3a no FBS, and (D) ATSP-7041 no FBS. Data shown are a representative experiment from more than 3 independent experiments.