Figure 6. Lumican-induced cell apoptosis can be overcome by overexpression of HIF-1α/ΔODD.
A, Left: MDA-PATC50 and MDA-PATC53 cells were treated with lumican (2μg/mL) for 24 hours in low-glucose, 0.5% FBS DMEM medium, and the live and dead cells were measured using a live/dead assay. The results shown are the average from three independent experiments. Middle: cell lysates were subjected to cell death detection ELISA kit (upper) and Western blotting with the indicated antibodies (lower). Right: Western blotting analysis of PARP and cleaved PARP. B, MDA-PATC53 cells were transfected with HIF-1α/ΔODD for 24 hours and treated with lumican (2μg/mL) for another 24 hours. Left: live/dead cell assay was performed. Middle: cell lysates were subjected to cell death detection ELISA assay. Right: Western blotting with the indicated antibodies. C, schematic model depicts how lumican inhibits glycolysis and induces apoptosis through the EGFR/Akt/HIF-1α signaling pathway.