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. Author manuscript; available in PMC: 2015 Jun 11.
Published in final edited form as: Nature. 2014 Sep 28;516(7530):263–266. doi: 10.1038/nature13769

Extended Data Figure 2. RNA cleavage is marginally stimulated by di- and trideoxyribonucleotide PAMmers.

Extended Data Figure 2

Cleavage reactions contained ~1 nM 5′-radiolabelled target ssRNA and no PAMmer (left), 100 nM 18-nt PAMmer (second from left), or 1 mM of the indicated di- or tri-nucleotide (remaining lanes). Reaction products were resolved by 12% denaturing polyacrylamide gel electrophoresis (PAGE) and visualized by phosphorimaging.