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. Author manuscript; available in PMC: 2014 Dec 8.
Published in final edited form as: Science. 2014 Mar 28;343(6178):1516–1520. doi: 10.1126/science.1251285

Fig. 2. The PDF negative clock neurons exert independent control over free-running activity rhythms.

Fig. 2

(A and B) Scatter plots of the predominant free-running periods of rhythmic flies overexpressing DBTS, SGGHypo or DBTL only in the PDF negative neurons (driven by Clk-GAL4/Pdf-GAL80) of wild-type flies (A) or Pdfr- mutants (B). Kruskal-Wallis one-way ANOVA reveals a significant difference among groups in both (A) and (B) (p < 0.0001 for both). (C) Scatter plots of the predominant free-running periods of rhythmic flies with different compositions of PDF positive and negative clock neurons. Specific genotypes are: per+, Pdf>DBTS for “Fast PDF+, Norm PDF-”, per01, Pdf>PER for “per+ PDF+, per01 PDF-”, and per01, Pdf>PER+DBTS for “Fast PDF+, per01 PDF-”. (D to F) Representative actograms (upper panels) and χ-square periodograms (lower panels) of individual flies with different compositions of PDF positive and negative neurons under constant darkness. Genotypes are as follows: (D) per+, Pdf>DBTS, (E) per01, Pdf>PER, and (F) per01, Pdf>PER+DBTS.