Figure 1.
cmk-1 mutants exhibit defects in negative thermotaxis and isothermal tracking behaviors.
A. Thermotaxis behaviors exhibited at ambient temperatures (T) relative to cultivation temperature (Tc). IT – isothermal tracking behavior.
B. Thermotaxis bias of the indicated strains on a spatial thermal gradient. cmk-1 cDNA was expressed specifically in AFD and AWC under the ttx-1 and ceh-36 promoters, respectively. Bars (± SEM) are the average of 10 independent assays of 15 animals each. Wild-type and mutant strains were examined together on multiple days. *** indicates different from wild-type at P<0.001; ### indicates different from cmk-1(oy21) at P<0.001 (ANOVA and Bonferroni post-hoc corrections).
C-D. Average track numbers (left) and lengths (right) of strains grown at the indicated temperatures. Animals grown at 25°C do not track well (Y.Y. and H.B., unpublished observations). Bars (± SEM) are the average of 8-10 independent assays of 15 animals each. Wild-type and mutant strains were examined together on multiple days. ** and *** indicate different from wild-type at P<0.01 and 0.001, respectively; ## and ### indicate different from cmk-1(oy21) at P<0.01 and 0.001, respectively (Kruskal-Wallis nonparametric ANOVA and Bonferroni post-hoc corrections). See Figure S1 for average tracking temperatures.