Figure 3. Macrophage NDST1 Inactivation Increases Foam Cell Conversion.
Oil Red O staining of peritoneal macrophages collected after 12-weeks HFC diet feeding (A). Oil Red O positive peritoneal macrophages (B) and cholesterol ester levels (C) in peritoneal macrophages collected after 12-weeks HFC diet feeding (n = 3/group). Cholesterol ester levels in BMDM after a 24h incubation with 0 or 50 µg/ml of LDL, agLDL and oxLDL (n = 3) (D). Cholesterol ester levels in BMDM after a 24h incubation with different concentrations of agLDL (n = 3) (E). Cholesterol ester levels in BMDM after a 24h incubation with 0 or 50 µg/ml of LDL and agLDL in the presence or absence of 100 ng/ml LPS (n = 3) (F). Binding at 4°C or uptake at 37°C for 1h of DiD-agLDL in BMDMs (n = 3) (G). Cholesterol efflux in BMDMs after 24h incubation with 10 µg/ml apoAI or 50 µg/ml HDL (n = 3) (H). Cholesterol ester content in BMDMs after cholesterol efflux with 10 µg/ml apoAI or 50 µg/ml HDL (n = 3) (I). Shown are mean ± SEM, *p<0.05 and **p<0.01.