Figure 1. Schematics of protein barcoding methods.
a, Collective barcoding via ribosome display. A short synthetic barcoding sequence is joined to the 5’ end of DNA templates via PCR. PRMC complexes are formed via ribosome stalling triggered by a C-terminal E. coli SecM peptide. Displayed proteins bearing a C-terminal Flag tag are separated from the ribosomes by an E. coli TolA spacer domain. b, Individual barcoding via a HaloTag-mediated conjugation of proteins to a 220-base pair (bp) double-stranded barcoding DNA with a HaloTag ligand modification (black triangle). Modifications are introduced to barcoding DNAs by PCR with modified primers.