Figure 4.
Effect of SH on the expression of lasI and rhlI. (A) RT-PCR of the lasI,rhlI and rpoD genes. The lanes from right to left were as follows: Marker, Control, 512 μg/ml (1 × MIC) SH, 256 μg/ml (1/2 × MIC) SH, 128 μg/ml (1/4 × MIC) SH, 64 μg/ml (1/8 × MIC), and 64 μg/ml (1 × MIC) AZM. (B) Quantitative RT-PCR of the lasI. (C) Quantitative RT-PCR of the rhlI. Expression of the house-keeping gene, rpoD, was used as the internal control for each sample. The drug concentration of treatments was as follows: 512 μg/ml (1 × MIC) SH, 256 μg/ml (1/2 × MIC) SH, 128 μg/ml (1/4 × MIC) SH, 64 μg/ml (1/8 × MIC), and 64 μg/ml (1 × MIC) AZM.