FIGURE 7:
Loss of specificity by concomitant knockdown of vps-33.1 and vps-33.2 leads to suppression of the sand-1 phenotype. (A) YP170-GFP uptake in sand-1(ok1963); vps-33.1(RNAi); vps-33.2(RNAi) oocytes (see legend to Figure 1 for detailed description). (B) Yolk granule size is reduced in sand-1(ok1963); vps-33.1+2(RNAi) worms (p = 7.6E-9 compared with sand-1; for detailed description, see Materials and Methods). (C) sand-1(ok1963); vps-33.1+2(RNAi) worms show a partial suppression of sand-1 defects (compared with wild-type and sand-1 shown above). (D) Profile plot across vps-33.1+2(RNAi) guts (see Figure 2G for description and comparison to wild-type and sand-1 worms). (E) RAB-5 vesicles in sand-1; vps-33.1+2 double-knockdown worms are wild-type size. Plots show 25th–75th percentile box, with median and whiskers indicating minimal and maximal values (the maximal value for sand-1, vps-33.2 was shortened for clarity). Vesicles in sand-1 are significantly larger than in wild type (p = 5.7E-31), sand-1; vps-33.2 vesicles are bigger than sand-1 alone (p = 1.6E-6), whereas sand-1; vps-33.1 (p = 0.06) and sand-1; vps-33.1+2 (p = 0.14) are the same as wild type.