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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Transl Res. 2014 May 24;164(5):359–365. doi: 10.1016/j.trsl.2014.05.011

Figure 2. In vitro demonstration of intercellular tumor-tumor and tumor-stromal interaction via TnTs in osteosarcoma.

Figure 2

TnTs formed between (A) K7M2 murine osteosarcoma cells; (B) K7M2 and MC3T3 murine osteoblast cells; and (C) Mg63.2 human OS cells and hFOB1.19 human OB cells. Cells were labeled using the lipophilic dyes DiI (red) and DiO (green) per the manufacturer’s protocol and per our prior demonstration using these dyes to visualize TnTs with fluorescence microscopy[4]. In cases of co-culture, cells were cultured in a 1:1 ratio.