Figure 6.
L11 is not regulated by transcription or protein stability and the total level of L11 does not change under growth inhibitory conditions. (A) WI-38 cells were serum-starved for the indicated time points and the L11 mRNA levels were analyzed by Northern blotting with a DNA probe containing the entire L11- or actin-coding sequences. (B) WI-38 cells were treated with 10 μg/ml puromycin for 60 min followed by collecting cells at indicated time points and analyzed by Western blotting. (C) WI-38 Cells were collected by trypsinization and lysed directly with 2% SDS–DTT protein gel loading buffer and the cell lysates were separated by SDS–PAGE followed by Western blotting.