Figure 4.
Inhibition of PPARγ through PGF2α diminishes the inhibitory effect of troglitazone and 15d-PGJ2 on MMP-9 expression and gelatinolytic activity in VSMCs. Western blot (A) and gelatin zymography (B) on supernatants of human VSMCs after treatment for 24 hours with PMA (50 mg/L), troglitazone (10 μmol/L) (trogl), or 15d-PGJ2 (10 μmol/L) in the presence or absence of 200 nmol/L/L PGF2α. PGF2α alone had no effect on MMP-9 gelatinolytic activity. Similar results were seen in 3 independent experiments.