FIGURE 7. PKCδ and PKCβ are not involved in VEGF-induced PKD activation.
A, BAEC were pretreated with PMA (500 nm) for 24 h and then exposed to VEGF (25 ng/ml) or PMA (500 nm) for 15 min. B–D, BAEC were pretreated with the PKCδ inhibitor Rotterlin (5μm, B) or classical PKC inhibitor Go6976 (100 nm, B) and PKCβ inhibitor LY379196 (100 nm, D) for 30 min and then exposed to VEGF (25 ng/ml) for 15 min. PKD activation and expression in cell lysates were determined by Western blot analysis as described in the legend for Fig. 1. Representative immunoblots from three independent experiments are shown. p-PKD(S744/748), the PKD phosphorylated at Ser-744 and Ser-748; DMSO, Me2SO.