FIG. 3.
Transcription of sycB originates at a promoter upstream of ysaU. (A) Schematic of the ysa locus encompassing the sycB promoter region. The approximate locations of primers KW114 and KW115 are indicated. (B) RT-PCR was performed with primers KW114 and KW115 and cDNA generated from 2 μg of total RNA that was isolated from JB580v as described in the text; 20% of the reaction was loaded on a 1.2% agarose gel and stained with ethidium bromide. Templates for the PCR are listed above each lane and were as follows: gDNA, genomic DNA; +RT, products from cDNA synthesis reaction with Superscript III added; no RT, products from cDNA synthesis reaction with no Superscript III; nt, no DNA or cDNA added.