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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Immunol. 2014 Jul 28;193(5):2405–2415. doi: 10.4049/jimmunol.1302798

Figure 3. mTOR is necessary for macrophage autophagy.

Figure 3

Primary peritoneal macrophages were subjected to RNAi using Non-target (NT) or mTOR siRNA. After 72 h, cells were exposed to LPS (100ng/mL) for 30 minutes. Total cellular lysate (10μg per lane) was then isolated and analyzed for the expression of mTOR, ATG7, ATG5-12, LC3B, and tubulin by immunblot (representative blot of n=3 independent experiments; N=3 mice total for all 3 experiments combined).