TABLE 4.
Limiting dilution experiments for different NMO-IgG populations
Limiting dilution (in ng/μl) of total NMO-IgG (NMO-IgG), NMO-IgG not binding D69H expressing cells (NMO-IgG D69H(−)), and NMO-IgG binding D69H-AQP4 expressing cells (NMO-IgG D69H(+)), tested on WT-AQP4 (WT) and D69H-AQP4 (D69H) expressing cells. Note that NMO-IgG D69H (+) represent 2% of total NMO-IgG, and recognizes WT and D69H with the same affinity.
| Cell line | Limiting concentration (ng/μl) |
|
|---|---|---|
| WT | D69H | |
| % | ||
| NMO-IgG | 0.2 | 10 (2) |
| NMO-IgG D69H(−) | 0.2 | 83 (0.2) |
| NMO-IgG D69H(+) | 0.2 | 0.2 (100) |