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EPR detection of the nitroxides formed from the spin trap EMPO (A) and the hydroxylamine spin probe TMT-H (B) in a xanthine oxidase O2• generating system (XO+X, 1 mU/ml xanthine oxidase plus 50 μM xanthine). Graphs show typical time scans of nitroxide accumulation followed by low field component of EPR spectrum (insert). Analysis of EPR sensitivity shows that Bruker EMX spectrometer equipped with SHQ microwave cavity has detection limit of 1 nM nitroxide.
