Figure 1. Om45 is exposed to the intermembrane space and its biogenesis involves Tom20 and Tom22.
A WT and mutant mitochondria were analysed by blue native electrophoresis and immunodetection with the indicated antisera.
B, C [35S]Om45 was imported into WT and mutant mitochondria. Samples were treated with proteinase K and analysed by blue native electrophoresis (B) or SDS–PAGE (C) and autoradiography.
D WT mitochondria or mitoplasts (+ swelling) were treated with proteinase K as indicated. Proteins were analysed by SDS–PAGE and immunodetection.
E WT and Om45His mitochondria or mitoplasts were incubated with anti-His antisera as indicated. Om45 complexes were detected by blue native electrophoresis and immunodetection.