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Protein binding pattern of Bim in resting and activated T cells. Lysates of resting or activated T cells were immunoprecipitated with rat anti-Bim (12) or with a control rat antibody that reacts with CD44. Immunoprecipitates (IP) with a cell equivalency of 22 × 106 per lane, together with post-IP supernatants (sup) with a cell equivalency of 4 × 106 per lane, were analyzed by Western blotting to evaluate the associations of Bim with Bcl-2, Bcl-x, LC8, Bax, or Bak.
