Figure 4.
rpl10 morphants display normal cell proliferation and increased apoptosis in the brain. (A and B) Whole-mount phospho-histone H3 staining for proliferating cells (M-phase marker) in control and rpl10 morphants at 2 dpf (lateral views). (C) Quantification of phospho-histone H3-positive cells from 20 embryos each (control embryos or embryos injected with rpl10 MO). Data are represented as the mean ± SEM. n.s., nonsignificant (two-tailed t-test comparisons between sb-MO-injected and rescued embryos). (D and E) TUNEL staining for apoptotic cells in control and rpl10 morphants at 2 dpf (lateral views). (F) Quantification of TUNEL staining intensities from 20 embryos each (control embryos or embryos injected with rpl10 sb-MO). Data are represented as the mean ± SEM. ***P < 0.0001 (two-tailed t-test comparisons between MO-injected and rescued embryos); similar results were obtained with the rpl10 tb-MO. Bars, 250 μm.