Figure 6. MiR-497 directly inhibited the expression of FGF2 and FGFR1 by binding to the 3′-UTR.
(A) Dual luciferase assay showed that FGF2 and FGFR1 were direct targets of miR-497. Cells were transfected with luciferase vectors containing either the wild-type or mutated binding site for miR-497 in FGF2 (left) or FGFR1 (right). The relative luciferase activity of cells co-transfected with vectors containing wild-type binding sequences along with miR-497 mimics was significantly decreased compared with that of cells co-transfected with mutated binding sequence vectors and mimics. Luciferase activities were also reduced compared with cells transfected with vectors containing wild-typed binding sequences and mimics control, as well as cells with mutant binding sequence vectors and mimics control. Data are displayed as the mean ± SD (*P < 0.05). (B) Protein expression levels of FGF2 and FGFR1 were detected by western blot.