Table 3.
Steady-state mechanical properties of skinned myocardial fibres isolated from NTG and TG hearts
| Group | Fmin (mN mm−2) | Fmax (mN mm−2) | nH | pCa50 |
|---|---|---|---|---|
| –PKA | ||||
| NTG | 0.61 ± 0.25 | 22.13 ± 1.73 | 4.10 ± 0.45 | 5.82 ± 0.02 |
| TGWT | 0.69 ± 0.20 | 21.74 ± 2.02 | 4.27 ± 0.56 | 5.81 ± 0.02 |
| TGS282A | 0.64 ± 0.21 | 21.41 ± 1.92 | 4.34 ± 0.66 | 5.82 ± 0.02 |
| +PKA | ||||
| NTG | 0.47 ± 0.20 | 21.31 ± 2.12 | 3.87 ± 0.47 | 5.71 ± 0.02* |
| TGWT | 0.52 ± 0.17 | 20.88 ± 1.71 | 4.11 ± 0.50 | 5.70 ± 0.02* |
| TGS282A | 0.51 ± 0.19 | 20.47 ± 1.84 | 3.79 ± 0.63 | 5.73 ± 0.03* |
Values are expressed as means ± s.e.m., from eight skinned fibres per group. Abbreviations: Fmin, Ca2+-independent force at pCa 9.0; Fmax, maximal Ca2+-activated force at pCa 4.5; nH, Hill coefficient of the force–pCa relationship; NTG, non-transgenic; pCa50, pCa required for half-maximal force generation; PKA, protein kinase A; TG, transgenic.
*
Significantly different from the corresponding non-PKA-treated group, P < 0.05.