Table 3.
Ethanol gavage increases the percentage of c-Fos-immunoreactive (c-Fos-IR) neurons that double-label with the orexin 2 receptor (OX2R) in the anterior paraventricular thalamus (aPVT), with no such effect detected in the posterior PVT (pPVT). It does not significantly affect double-labeling of c-Fos with the orexin 1 receptor (OX1R). Control = no gavage; Water = water gavage; Low Ethanol = 0.75 g/kg ethanol per gavage; High Ethanol = 1.50 g/kg ethanol per gavage.
| Brain Region | % c-Fos+OX1R Neurons | |||
| Control | Water | Low Ethanol | High Ethanol | |
| PVT | 3.5 ± 0.4 | 5.7 ± 0.4 | 5.3 ± 1.2 | 5.3 ± 0.9 |
| aPVT | 3.5 ± 0.8 | 5.2 ± 0.7 | 4.5 ± 1.4 | 4.5 ± 0.7 |
| pPVT | 3.1 ± 1.0 | 6.3 ± 1.3 | 6.4 ± 1.6 | 7.0 ± 1.6 |
| % c-Fos+OX2R Neurons | ||||
| PVT | 6.4 ± 0.7 | 5.9 ± 0.4 | 10.1 ± 1.1**+ | 13.0 ± 1.0***+++ |
| aPVT | 6.5 ± 1.3 | 5.6 ± 1.2 | 15.9 ± 1.1***+++ | 18.0 ± 1.2***+++ |
| pPVT | 6.4 ± 1.8 | 5.7 ± 1.4 | 5.0 ± 2.5 | 5.7 ± 1.3 |
***
p < 0.001 vs. Water group;
**
p < 0.01 vs. Water group;
*
p < 0.05 vs. Water group;
+++
p < 0.001 vs. Control group;
++
p < 0.01 vs. Control group;
+
p < 0.05 vs. Control group.