Figure 2.
Multiple miRNAs downregulate human FOXP2 expression. (A) Representative Western blot images and quantitative results showing that miR-9, miR-19b, miR-140-5p, miR-200a, let-7a, miR-129-5p, miR-582-5p, and miR-892a downregulated human FOXP2 protein expression when transfected into HEK293 cells. (B and C) The downregulatory effects of let-7a, miR-9, and miR-129-5p were dosage-dependent. (D) Let-7a, miR-9, and miR-129-5p downregulated FOXP2 mRNA expression in similar transfection experiments as measured by qRT-PCR. For both Western blot and qRT-PCR, Actin was used as an internal control; a miRNA mimic with a scrambled sequence (Ambion) was used as a negative control; and a siRNA against FOXP2 siFOXP2 was used as a positive control. Transfection was performed at least three times (n = 3) for each miRNA, and quantifications were averaged from the three transfections, two Western blot or two qRT-PCR experiments per transfection. *p < 0.05, **p < 0.01, and ***p < 0.001.