TABLE 4.
Genes induced by cAMP (forskolin and dbcAMP)
Classification of the genes induced by a 6-h treatment with PACAP (100 nM), forskolin (250 nM), dbcAMP (10 mM), and/or NGF (100 ng/ml). Transcripts were classified in decreasing order of magnitude of induction. Some transcripts with a ratio above 1.5 were not included in a particular category for a given treatment, if the data did not also satisfy microarray quality criteria (quality index >0.3). Bold characters indicate genes further investigated by real-time PCR as reported in Table 6.
| Gene Name | Unigene Number | GenBank ID | PACAP
|
Forskolin
|
dbcAMP
|
NGF
|
||||
|---|---|---|---|---|---|---|---|---|---|---|
| Av | S.E.M. | Av | S.E.M. | Av | S.E.M. | Av | S.E.M. | |||
| Forskolin | ||||||||||
| Ferric-chelate reductase 1 (Frrs1) | Mm 66293 | C86591 | 1.7 | 0.3 | 2.9 | 1.1 | 2.2 | 0.7 | 1.2 | 0.2 |
| Bone morphogenetic protein 6 (Bmp6) | Mm 28622 | C76305 | 3.1 | 0.7 | 2.6 | 0.4 | 2.1 | 0.2 | 1.2 | 0.1 |
| Chromogranin B (Chgb) | Mm 255241 | 367410.00 | 1.2 | 0.3 | 2.6 | 0.3 | 2.2 | 0.2 | 0.8 | 0.1 |
| Peptidylglycine α-amidating monooxygenase (Pam) | Mm 5121 | 482270.00 | 1.2 | 0.4 | 2.2 | 0.3 | 1.9 | 0.3 | 0.7 | 0.1 |
| Fibrinogen, βpolypeptide (Fgb) | Mm 30063 | 552321.00 | 0.9 | 0.0 | 2.0 | 0.1 | 1.4 | 0.2 | 0.8 | 0.0 |
| Methionine adenosyltransferase IIα (Mat2a) | Mm 29815 | AW542928 | 1.6 | 0.3 | 2.0 | 0.2 | 2.1 | 0.3 | 0.9 | 0.1 |
| Leucine rich repeat containing 3 (Lrrc3) | Mm 133301 | 316779.00 | 0.8 | 0.1 | 1.9 | 0.1 | 1.5 | 0.3 | 0.9 | 0.0 |
| X-box binding protein 1 (Xbp1) | Mm 22718 | AU040737 | 1.9 | 0.2 | 1.9 | 0.1 | 1.7 | 0.2 | 1.4 | 0.1 |
| Gene trap ROSA b-geo 22 (Gtrgeo22) | Mm 22632 | AW544177 | 1.6 | 0.2 | 1.8 | 0.0 | 1.9 | 0.1 | 1.1 | 0.1 |
| 70-kDa Heat shock protein 5 (glucose-regulated protein) (Hspa5) | Mm 330160 | AW537792 | 1.5 | 0.1 | 1.7 | 0.2 | 1.4 | 0.2 | 0.9 | 0.1 |
| Frizzled homolog 7 (Drosophila) (Fzd7) | Mm 297906 | AW537516 | 1.0 | 0.1 | 1.7 | 0.3 | 1.4 | 0.2 | 1.0 | 0.1 |
| 13 days embryo heart cDNA, RIKEN full-length enriched library, clone:D330042P15 | Mm 417626 | AU020988 | 1.2 | 0.1 | 1.6 | 0.3 | 1.3 | 0.2 | 1.1 | 0.1 |
| UDP-GlcNAc: βGal β-1,3-N-acetylglucosaminyltransferase 1 (B3gnt1) | Mm 258094 | AU024115 | 1.3 | 0.1 | 1.6 | 0.0 | 1.4 | 0.1 | 1.0 | 0.1 |
| RIKEN cDNA 9630050M13 gene (9630050M13Rik) | Mm 23044 | AA409679 | 1.3 | 0.1 | 1.5 | 0.1 | 1.6 | 0.2 | 1.0 | 0.1 |
| Transmembrane protein 16F (Tmem16f) | Mm 38087 | AW553814 | 1.3 | 0.2 | 1.5 | 0.1 | 1.5 | 0.3 | 0.8 | 0.1 |
| dbcAMP | ||||||||||
| Ras homolog gene family, member Q (Rhoq) | Rn0.4169 | AW557645 | 1.4 | 0.2 | 1.4 | 0.1 | 1.9 | 0.2 | 1.1 | 0.1 |
| Homer homolog 2 (Drosophila) (Homer2) | Mm 228 | AA407944 | 1.5 | 0.2 | 1.5 | 0.1 | 1.7 | 0.2 | 1.3 | 0.1 |
| RAB6, member RAS oncogene family (Rab6) | Mm 28650 | AW552337 | 1.5 | 0.3 | 1.3 | 0.1 | 1.7 | 0.2 | 1.2 | 0.2 |
| Protein tyrosine phosphatase, receptor type, F (Ptprf) | Mm 29855 | AW548091 | 1.2 | 0.2 | 1.3 | 0.1 | 1.6 | 0.2 | 0.9 | 0.1 |
| RIKEN cDNA 1700020D05 gene (1700020D05Rik) | Mm 20071 | AW558842 | 1.5 | 0.6 | 1.4 | 0.3 | 1.6 | 0.2 | 0.9 | 0.1 |
| Phosphoribosyl pyrophosphate amidotransferase (Ppat) | Mm 202337 | AA408689 | 1.0 | 0.1 | 1.6 | 0.1 | 1.6 | 0.2 | 0.9 | 0.1 |
| CAMP-regulated phosphoprotein 19 (Arpp19) | Mm 247837 | AW559096 | 1.4 | 0.1 | 1.4 | 0.1 | 1.6 | 0.1 | 0.9 | 0.1 |
| Single-stranded DNA binding protein 3 (Ssbp3) | Mm 195635 | AW551939 | 1.3 | 0.1 | 1.3 | 0.1 | 1.6 | 0.1 | 1.1 | 0.1 |
| Protein kinase, cAMP dependent regulatory, type I, α (Prkar1a) | Mm 30039 | AW555666 | 1.5 | 0.2 | 1.5 | 0.1 | 1.6 | 0.1 | 1.2 | 0.1 |
| T 3-monooxygenase/w 5-monooxygenase activation protein, z polypeptide (Ywhaz) | Mm 3360 | AW544726 | 1.2 | 0.1 | 1.3 | 0.1 | 1.5 | 0.1 | 1.1 | 0.1 |