TABLE 2.
Genes induced by at least one factor, PACAP, forskolin, dbcAMP, and/or NGF
Classification of the genes induced by a 6-h treatment with PACAP (100 nM), forskolin (25 μM), dbcAMP (1 mM), and NGF (100 ng/ml). Transcripts were classified in decreasing order of magnitude of induction. Some transcripts with a ratio above 1.5 were not included in a particular category for a given treatment, if the data did not also satisfy microarray quality criteria (quality index >0.3). Bold characters indicate genes further investigated by real-time PCR as reported in Table 6.
| Gene Name | Unigene Number | GenBank ID | PACAP
|
Forskolin
|
dbcAMP
|
NGF
|
||||
|---|---|---|---|---|---|---|---|---|---|---|
| Av | S.E.M. | Av | S.E.M. | Av | S.E.M. | Av | S.E.M. | |||
| PACAP Forskolin dbcAMP NGF | ||||||||||
| Ornithine decarboxylase, structural 1 (Odc1) | Mm 34102 | AU020132 | 9.8 | 1.0 | 4.5 | 0.1 | 3.2 | 0.2 | 3.9 | 0.4 |
| Villin 2 (Vil2) | Mm 277812 | AW558398 | 2.4 | 0.2 | 1.5 | 0.0 | 1.6 | 0.2 | 1.5 | 0.1 |
| HIV-1 Rev binding protein (Hrb) | Mm 6461 | C81336 | 2.2 | 0.2 | 1.7 | 0.1 | 1.8 | 0.1 | 1.7 | 0.2 |
| PACAP Forskolin dbcAMP | ||||||||||
| Regulator of G-protein signaling 2 (Rgs2) | Mm 28262 | AU023169 | 10.0 | 3.4 | 3.1 | 1.2 | 1.6 | 0.2 | 1.4 | 0.1 |
| Immediate early response 3 (Ier3) | Mm 25613 | C87164 | 8.8 | 1.8 | 3.7 | 1.7 | 2.0 | 0.3 | 1.4 | 0.1 |
| Antizyme inhibitor 1 (Azin1) | Mm 250214 | AU016852 | 5.9 | 1.1 | 4.9 | 0.3 | 4.9 | 0.6 | 1.1 | 0.1 |
| MAP kinase-activated protein kinase 2 (Mapkapk2) | Mm 221235 | AW557658 | 5.9 | 0.6 | 4.2 | 0.2 | 4.4 | 0.5 | 1.4 | 0.1 |
| ESTs | Mm 17000 | AU016391 | 4.9 | 0.6 | 2.6 | 1.0 | 1.8 | 0.3 | 1.2 | 0.1 |
| Protein tyrosine phosphatase 4a1 (Ptp4a1) | Mm 374437 | AU022218 | 4.0 | 0.4 | 2.7 | 0.1 | 2.6 | 0.2 | 1.3 | 0.1 |
| Transcribed locus | Mm 401636 | AW548730 | 3.9 | 0.4 | 2.6 | 0.3 | 2.4 | 0.2 | 1.2 | 0.1 |
| RIKEN cDNA 4930431J08 gene (4930431J08Rik) | Mm 86986 | AW554859 | 3.6 | 0.3 | 2.1 | 0.1 | 1.9 | 0.3 | 1.1 | 0.1 |
| Growth arrest specific 1 (Gas1) | Mm 22701 | AW554898 | 3.5 | 0.4 | 2.5 | 0.4 | 2.3 | 0.4 | 0.9 | 0.1 |
| Prickle like 1 (Drosophila) (Prickle 1) | Mm 150314 | AU022455 | 3.5 | 0.3 | 2.3 | 0.2 | 2.2 | 0.3 | 1.2 | 0.1 |
| Plexin A2 (Plxna2) | Mm 392736 | AW551294 | 3.2 | 0.4 | 1.8 | 0.3 | 1.4 | 0.1 | 1.1 | 0.1 |
| RIKEN cDNA 1500041J02 gene (1500041J02Rik) | Mm 281019 | AU020735 | 2.9 | 0.4 | 2.6 | 0.3 | 2.1 | 0.4 | 1.3 | 0.1 |
| Eukaryotic translation initiation factor 1 (Eif1) | Mm 13886 | AW545196 | 2.5 | 0.2 | 1.7 | 0.1 | 1.9 | 0.2 | 1.2 | 0.0 |
| Activating transcription factor 4 (Atf4) | Mm 641 | AW550463 | 2.2 | 0.2 | 1.9 | 0.1 | 1.9 | 0.2 | 1.2 | 0.1 |
| ATPase, class VI, type 11A (Atp11a) | Mm 257837 | AU024208 | 2.1 | 0.2 | 1.6 | 0.2 | 1.7 | 0.2 | 1.4 | 0.1 |
| Transcribed locus | Mm 188460 | AW543519 | 2.1 | 0.2 | 1.6 | 0.1 | 1.8 | 0.2 | 1.1 | 0.1 |
| RIKEN cDNA 1810013L24 gene (1810013L24Rik) | Mm 390868 | AU024712 | 2.0 | 0.3 | 1.8 | 0.3 | 1.8 | 0.2 | 1.4 | 0.1 |
| Ubiquitin-conjugating enzyme E2L 3 (Ube213) | Mm 3074 | AW551421 | 2.0 | 0.3 | 2.0 | 0.2 | 2.0 | 0.2 | 0.9 | 0.1 |
| DNA segment, Chr 3, University of California at Los Angeles 1 (D3Ucla1) | Mm 29702 | AW539563 | 1.9 | 0.2 | 1.6 | 0.1 | 2.0 | 0.3 | 1.1 | 0.1 |
| Tubulin, β2b (Tubb2b) | Mm 379227 | AU020799 | 1.9 | 0.2 | 1.7 | 0.1 | 1.6 | 0.1 | 1.1 | 0.1 |
| RIKEN cDNA 2610024B07 gene (2610024B07Rik) | Mm 24685 | AW536239 | 1.7 | 0.2 | 1.6 | 0.0 | 1.6 | 0.1 | 1.3 | 0.1 |
| Nuclear distribution gene E-like homolog 1 (Aspergillus nidulans) (Ndel1) | Mm 31979 | AU042890 | 1.7 | 0.2 | 1.6 | 0.0 | 1.6 | 0.1 | 1.4 | 0.1 |
| Pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3) | Mm 12775 | AW556440 | 1.7 | 0.2 | 2.0 | 0.2 | 2.0 | 0.2 | 0.9 | 0.1 |
| Tyrosine hydroxylase (Th) | Mm 1292 | C85951 | 1.6 | 0.2 | 1.8 | 0.1 | 1.9 | 0.3 | 1.2 | 0.1 |
| PACAP Forskolin NGF | ||||||||||
| Arsenic (+3 oxidation state) methyltransferase (As3mt) | Mm 28566 | AU020528 | 6.0 | 1.2 | 1.9 | 0.4 | 1.4 | 0.2 | 1.9 | 0.1 |
| RIKEN cDNA 5830411E10 gene (5830411E10Rik) | Mm 196290 | AU019102 | 2.4 | 0.3 | 1.7 | 0.3 | 1.4 | 0.2 | 1.7 | 0.2 |
| Annexin A2 (Anxa2) | Mm 238343 | AW551165 | 2.4 | 0.3 | 1.6 | 0.1 | 1.4 | 0.2 | 1.5 | 0.1 |
| CAMP responsive element binding protein 3-like 2 (Creb312) | Mm 169929 | AU042737 | 2.1 | 0.4 | 1.8 | 0.3 | 1.4 | 0.3 | 1.7 | 0.2 |
| PACAP Forskolin | ||||||||||
| Sex comb on midleg homolog 1 (Scmh1) | Mm 388903 | C86855 | 5.0 | 0.5 | 2.2 | 0.5 | 2.1 | 0.2 | 1.1 | 0.1 |
| Plasminogen activator, tissue (Plat) | Mm 154660 | AU020998 | 4.1 | 1.1 | 3.2 | 0.9 | 2.0 | 0.5 | 1.2 | 0.1 |
| Hect domain and RLD 4 (Herc4) | Mm 234437 | AW553563 | 4.0 | 0.7 | 1.8 | 0.3 | 1.5 | 0.1 | 1.0 | 0.1 |
| LIM domain only 1 (Lmo1) | Mm 360145 | AU015284 | 3.3 | 0.7 | 1.7 | 0.4 | 1.3 | 0.1 | 1.2 | 0.1 |
| EH-domain containing 4 (Ehd4) | Mm 132226 | AU044505 | 3.0 | 0.3 | 1.7 | 0.2 | 1.5 | 0.2 | 1.4 | 0.1 |
| Heat shock protein 8 (Hspb8) | Mm 21549 | AU018999 | 2.5 | 0.3 | 1.6 | 0.1 | 1.5 | 0.1 | 1.2 | 0.1 |
| RIKEN cDNA 2410019A14 gene | Mm 24586 | AW555539 | 2.4 | 0.3 | 1.8 | 0.2 | 1.5 | 0.2 | 1.0 | 0.1 |
| Inhibitor of DNA binding 2 (Id2) | Mm 34871 | AW548400 | 2.2 | 0.2 | 1.6 | 0.0 | 1.5 | 0.1 | 1.4 | 0.1 |
| 70-kDa Heat shock protein 5 (glucose-regulated protein) (Hspa5) | Mm 330160 | AW555441 | 1.9 | 0.4 | 1.6 | 0.1 | 1.6 | 0.2 | 0.9 | 0.1 |
| PACAP dbcAMP | ||||||||||
| Cytoplasmic polyadenylation element binding protein 4 (Cpeb4) | Mm 339792 | AU015651 | 1.8 | 0.3 | 1.4 | 0.1 | 1.8 | 0.1 | 1.1 | 0.2 |
| Mitogen-activated protein kinase 6 (Mapk6) | Mm 18856 | AU042016 | 1.6 | 0.1 | 1.4 | 0.0 | 1.6 | 0.2 | 1.2 | 0.1 |
| 3-monooxygenase/w 5-monooxygenase activation protein, γ polypeptide (Ywhag) | Mm 233813 | AU014738 | 1.5 | 0.2 | 1.3 | 0.1 | 1.7 | 0.2 | 1.0 | 0.1 |
| Forskolin dbcAMP | ||||||||||
| Amylo-1,6-glucosidase, 4-α-glucanotransferase (Agl) | Mm 237099 | C77182 | 1.5 | 0.1 | 2.8 | 0.4 | 2.3 | 0.6 | 0.9 | 0.1 |
| Cysteine and glycine-rich protein 1 (Csrp1) | Mm 196484 | AA408841 | 2.7 | 0.4 | 2.7 | 0.1 | 2.9 | 0.3 | 1.0 | 0.1 |
| Tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2 (Tanc2) | Mm 22501 | AU022723 | 1.7 | 0.3 | 2.3 | 0.3 | 2.5 | 0.4 | 1.2 | 0.1 |
| RIKEN cDNA 2810407C02 gene (2810407C02Rik) | Mm 270950 | AW537092 | 1.7 | 0.2 | 2.0 | 0.1 | 1.6 | 0.2 | 1.0 | 0.1 |
| GATA binding protein 2 (Gata2) | Mm 272747 | AW538547 | 1.0 | 0.1 | 1.9 | 0.1 | 1.9 | 0.2 | 0.9 | 0.1 |
| Nucleophosmin 1 (Npm1) | Mm 6343 | AW553526 | 1.5 | 0.2 | 1.9 | 0.1 | 1.8 | 0.2 | 0.9 | 0.1 |
| Growth factor receptor bound protein 10 (Grb10) | Mm 273117 | AW556824 | 1.2 | 0.1 | 1.7 | 0.1 | 1.9 | 0.2 | 0.9 | 0.1 |
| Solute carrier family 31, member 1 (Slc31a1) | Mm 248637 | AU016967 | 1.4 | 0.1 | 1.6 | 0.1 | 1.7 | 0.2 | 0.7 | 0.1 |
| PACAP NGF | ||||||||||
| Serine (or cysteine) peptidase inhibitor, clade B, member 1a (Serpinb1a) | Mm 20144 | AW549049 | 5.6 | 1.5 | 1.2 | 0.2 | 1.0 | 0.1 | 2.6 | 0.7 |
| Activated leukocyte cell adhesion molecule (Alcam) | Mm 288282 | AW549010 | 3.2 | 0.5 | 1.5 | 0.2 | 1.3 | 0.2 | 1.8 | 0.1 |
| Aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3) | Mm 451 | AW550812 | 2.4 | 0.2 | 1.4 | 0.1 | 1.2 | 0.1 | 1.7 | 0.1 |
| Cytochrome P450 oxidoreductase (Por) | Mm 3863 | AU016777 | 2.0 | 0.3 | 1.5 | 0.1 | 1.4 | 0.1 | 1.5 | 0.2 |
| Adenomatosis polyposis coli (Apc) | Mm 7883 | AW550666 | 2.0 | 0.2 | 1.4 | 0.2 | 1.4 | 0.2 | 1.8 | 0.2 |
| Methionine-tRNA synthetase 2 (mitochondrial) (Mars2) | Mm 19223 | AW539214 | 1.9 | 0.1 | 1.4 | 0.1 | 1.5 | 0.2 | 1.5 | 0.1 |
| Myeloid cell leukemia sequence 1 (Mcl1) | Mm 1639 | C81342 | 1.8 | 0.2 | 1.3 | 0.1 | 1.5 | 0.2 | 1.6 | 0.2 |
| Expressed sequence AU045404 (AU045404) | Mm 17853 | AU045358 | 1.7 | 0.1 | 1.2 | 0.1 | 1.2 | 0.1 | 4.4 | 0.5 |
| Plectin 1 (Plec1) | Mm 234912 | AU042599 | 1.7 | 0.1 | 1.2 | 0.1 | 1.3 | 0.1 | 1.6 | 0.1 |
| Aryl hydrocarbon receptor nuclear translocator-like (Arnt1) | Mm 12177 | AU045956 | 1.6 | 0.2 | 1.2 | 0.1 | 1.3 | 0.1 | 1.6 | 0.1 |
| Palladin, cytoskeletal associated protein (Palld) | Mm 29933 | AU045282 | 1.6 | 0.1 | 1.0 | 0.1 | 1.1 | 0.1 | 2.6 | 0.3 |
| Tax1 (human T-cell leukemia virus type I) binding protein 3 (Tax1bp3) | Mm 371656 | AW544713 | 1.6 | 0.1 | 1.3 | 0.1 | 1.2 | 0.1 | 1.7 | 0.1 |
| Forskolin NGF | ||||||||||
| Zinc finger, CCHC domain containing 18 (Zcchc18) | Mm 23671 | 615116.00 | 1.8 | 0.1 | 2.2 | 0.1 | 1.3 | 0.3 | 2.0 | 0.2 |
ESTs, expressed sequence tags.