FIG. 7.
Biochemical analysis after intermittent forskolin or PTHrP(7–34) treatment. Pellets received chondrogenic medium alone (ctrl) or were additionally stimulated for 6 h with either 2.5 nM PTHrP(1–34), 2.5 nM PTHrP(7–34), or 10 μM forskolin (forsk) before each medium change, respectively. (A, B) On day 42, pellets were harvested and proteoglycan (A) and DNA content (B) were determined. The values are given as the mean±SD of 5 [PTHrP(7–34)] or 10 (all other groups) independent experiments. (C) ALP enzyme activity was measured in culture supernatants conditioned for 2 days each. Values are given as the mean±SD of five to six independent experiments. *P≤0.05, **P≤0.01, Wilcoxon test.