FIG 7.
Size exclusion chromatography analysis of the secreted and purified K8 BDNF mutant. (A) (Top) Elution chromatogram of molecular size standards. (Bottom) Western blot of K8 sample eluates aligned by elution time with the molecular size standards. Samples were run under reducing conditions with (+) or without (−) glutaraldehyde (G.A.) cross-linking. (B) Western blot of secreted and purified K8 under both reducing and nonreducing SDS-PAGE conditions (with and without DTT) and with and without cross-linking (with and without G.A.). A slightly overexposed blot is presented to allow viewing of all BDNF species.