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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: Dev Biol. 2013 Sep 7;383(2):307–320. doi: 10.1016/j.ydbio.2013.08.019

Figure 5. Coronary plexus remodeling dejects in Tbx18-/- hearts.

Figure 5

(A,B) Stacked Z-plane confocal images of PECAM-1 (red) whole mount staining of E14.5 hearts. Two coronary stems (dashed yellow lines) form normally in Tbx18-/- hearts (B) compared to wild type hearts (A). (C,D) Once coronary blood flow is established at E13.5, vascular remodeling occurs. Continuous, branching channels for blood flow can be identified in the subepicardium of E14.5 wild type hearts (C, yellow dashed lines), whereas similar channels are difficult to find in Tbx18-/- hearts (D). Ventral views of E16.5 (E,F) hearts showing significant alterations in the pattern, and reductions in length and diameter (arrow) of the left descending coronary artery in mutant hearts. Note persistence of PECAM-1pos nodules in mutant hearts.