Fig. 6.
IL-6 did not affect proliferation in differentiating p21-/- neuronal progenitors. One thousand WT and p21-/- neuronal progenitor cells were plated in 24-well plates in triplicates, and kept in differentiating conditions for 8 days. A) Cells were co-stained for Tuj-1 and Ki-67. The graph depicts the percentage of Ki-67+ cells among Tuj-1+ neuroblasts. B) Cells were co-stained with GFAP and Ki-67. The graph depicts the percentage of Ki-67+ among GFAP+ cells. Counting was performed in triplicates (>500 cells per sample). Data are presented as a mean ± SEM, *, p<0.05. Three independent experiments were conducted, and representative results are shown.