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. Author manuscript; available in PMC: 2014 Sep 9.
Published in final edited form as: Mol Cell. 2010 Sep 24;39(6):925–938. doi: 10.1016/j.molcel.2010.08.011

Figure 5. MALAT1 Depletion Results in the Fragmentation of Cell Nuclei.

Figure 5

(A and C) MALAT1 RNA-FISH in U2OS (A) and EpH4 (C) cells that were transfected with control (scr-oligo; a–a′), human specific (A, AS1 and AS2; b–b′ and c–c′) or mouse-specific (C; AS4 and AS5; b–b′ and c–c′) MALAT1 antisense oligonucleotides (72 hr posttransfection). (B) In vivo Br-UTP transcription analyses in control

(Ba–Ba′) and MALAT1 antisense oligo-transfected (Bb–Bb′ and Bc–Bc′) HeLa cells. The bars in (A), (B), and (C) represent 10 μm. (D) Flow cytometric analyses revealed increased cell death (<2C DNA content) with increased G2/M population in MALAT1-depleted HeLa cells. See also Figure S5.