FIG. 4.
Digital images of fluorescence micrographs of mock-infected (A, i to iii; B, i; and C, i) and HSV-1-infected cells. (A) Vero cells were infected with the deletion mutant viruses RR1072 (deletion of UL34; A, iv and vi) or R5132 (deletion of UL31; A, x to xii) or rescued virus RR1072Rep (rescued UL34; A, vii to ix) or R5133 (rescued UL31; A, xiii and xiv). Cells were fixed at 18 h postinfection and stained with antibodies specific for H1 (red) and ICP8 (green). (B) Vero cells were infected with the deletion mutant virus RR1072 or R5132 or with wild-type HSV-1 KOS strain (B, ii and iii). Cells were fixed at 18 h postinfection and stained with antibodies specific for LAP2 (red) and ICP8 (green). (C) Hep2 cells were infected with the deletion mutant virus RR1072 or R5132 or with wild-type HSV-1 KOS strain (C, ii and iii). Cells were fixed at 18 h postinfection and stained with antibodies specific for lamin A/C (red) and ICP8 (green). In all cases, red- and green-channel images were merged to show the relationship between the staining patterns (merge column). Images were digitally enlarged to show detail (zoom column).