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MSC culture and characterization. (A) A representative plot shows the fibroblast-like morphology of cultured MSCs. (B–D) Representative flow cytometry plots show the expression of CD29, but not CD31 and CD45, in cultured MSCs. Left curves, isotype control; Right curves, antibodies. (E) Osteogenic differentiation capacity of MSCs confirmed by alizarin red S staining. (F) Adipogenic differentiation capacity of MSCs determined using oil red O staining. Magnification in A, E, and F: ×10.
