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. Author manuscript; available in PMC: 2015 Aug 5.
Published in final edited form as: Cell Metab. 2014 Jul 3;20(2):306–319. doi: 10.1016/j.cmet.2014.06.004

Figure 2. Acetyl-CoA: CoASH ratio is a determinant of histone acetylation in cancer cells.

Figure 2

(A) Molar concentrations of acetyl-CoA, CoASH, and ratio of acetyl-CoA: CoASH over 24 hours in LN229 cells, mean +/− SEM of triplicates. Result is representative of 3 independent experiments. (B) IL-3-dependent bax−/−bak−/− cells were cultured for 48 hours +/− glucose, +/− IL-3 as indicated. Acetyl-CoA and CoASH were measured and normalized to cell volume, mean +/− SD of triplicates. Significance as compared to Glc+IL-3+ samples (**, p<0.005, ***, p<0.0005) (C) Representative Western blot of acetylated histones upon incubation of isolated nuclei with varying concentrations of acetyl-CoA and CoASH. Total histones were stained by Ponceau. Data was quantified from four independent experiments with 25:0 value set to 1, mean +/− SEM. Repeated measures one-way ANOVA with post test for linear trend was performed for 25:10–25:100 values. Post test for linear trend significance for AcH4: H4, p<0.0001 (***); for AcH3: H3, p=0.0034 (**).