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. 2014 Sep 1;206(5):589–598. doi: 10.1083/jcb.201403096

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© 2014 Ziani et al.

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Figure 4. — Tethering DDB2 to chromatin recruits XPC through its N- and C-terminal extremities. (A, left) Schematic representation of YFP-DDB2^(1–427) and YFP-LacR-DDB2^(1–427). For clarity, the sizes of the YFP (238 aa) and LacR (367 aa) were omitted. (right) Proteins from whole cell extracts (15 µg) of U2OS17 expressing either YFP-DDB2^(1–427) or YFP-LacR-DDB2^(1–427) were resolved by SDS-PAGE followed by Western blotting using anti-GFP antibodies. (B–D) Recruitment of XPC (B), XPB (C), or flag-XPA (D) to tethered YFP-LacR-DDB2^(1–427). The values on the graphs represent the percentage of colocalization of each NER factor with YFP on the array based on three independent experiments with SD. Circles indicate LacO arrays. (E) Schematic representation of wild-type and deleted flag-XPC constructs. (F) Recruitment of flag-XPC constructs to tethered YFP-LacR-DDB2^(1–427). The values represent the percentage of colocalization of each Flag-XPC constructs with YFP on the array based on three independent experiments with SD.