Extended Data Figure 3. Effects of miR-34a over-expression using additional osteoclastic cre drivers.
a-d, 34a-Lys-Tg mice were generated using Lysozyme-cre driver. e-h, 34a-Ctsk-Tg mice were generated using Ctsk-cre driver. a,e, Bone marrow osteoclast differentiation assays. Left, mature miR-34a level (n=3); middle, TRAP mRNA expression (n=3); right, TRAP staining of differentiation cultures, quantification of mature osteoclast numbers per well in 24-well plates (black, n=3), and quantification of bone resorptive activity by calcium release from bone plate into culture medium (μM) (blue, n=6). b,f, Serum CTX-1 (2-month-old males; b, n=5, f, n=8). c,g, Trabecular BV/TV of proximal tibiae by μCT (2-month-old males; c, n=4; g, n=4). d,h, Histomorphometry of the distal femur and vertebrae in 2-month-old mice.